|
KMID : 0364820100460030291
|
|
Korean Journal of Microbiology
2010 Volume.46 No. 3 p.291 ~ p.295
|
|
|
Simple and Rapid Extraction of a Bacteriocin Produced by Streptococcus parauberis Z49 from Fermented Cultures
|
|
Park Hong-Je
Khang Yong-Ho
|
|
|
Abstract
|
|
|
A novel bacteriocin produced by Streptococcus parauberis Z49 strain was characterized and efficiently extracted from fermented cultures by use of aqueous two-phase systems. The nisin-like bacteriocin, which was active even after a heat treatment at 121°C for 15 min and in the broad pH range from 2 to 12, showed inhibition of bacterial growth of Micrococcus luteus, Lactobacillus spp., Lactobacillus fermentum, Enterococcus faecium, Listereia monocytogenes, and Pseudomonas fluorescens. Optimal conditions of PEG 600/Na2SO4 aqueous two-phase systems for the simple and rapid extraction of a
novel bacteriocin were determined to be PEG 600 15%, Na2SO4 30%, and NaCl 8%, where the bacteriocin was concentrated in PEG layer.
|
|
|
KEYWORD
|
|
|
aqueous two-phase system, bacteriocin, extraction, nisin, S. parauberis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|